Expression analysis of innate immunity related genes in the true/field blast resistance gene-mediated defence response

Debing Wang; Yonghua Qin; Jingluan Han; Ling Zhang; Xin Xu; Xuequn Liu; Chuntai Wang; Xinqiong Liu

doi:10.1080/13102818.2014.978664

Expression analysis of innate immunity related genes in the true/field blast resistance gene-mediated defence response

Biotechnol Biotechnol Equip. 2014 Nov 2;28(6):999-1007. doi: 10.1080/13102818.2014.978664. Epub 2014 Nov 18.

Authors

Debing Wang¹, Yonghua Qin¹, Jingluan Han², Ling Zhang³, Xin Xu¹, Xuequn Liu¹, Chuntai Wang¹, Xinqiong Liu¹

Affiliations

¹ Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities , Wuhan , Hubei , P.R. China.
² Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Science, South-Central University for Nationalities, Wuhan, Hubei, P.R. China; College of Life Science, South China Agricultural University, Guangzhou, Guangdong, P.R. China.
³ Agricultural Technology Extension and Service Center of Jingzhou City , Jingzhou , Hubei , P.R. China.

Abstract

Rice blast resistance (R) genes-mediated resistance response depends on various resistance-related genes involved in incompatible interactions. In this work, the expression profiles of innate rice immunity related genes were examined in the mediated resistance response of true/field resistance genes. Three sets of rice near-isogenic lines (NILs) were used: the resistant NILs carrying true resistance genes in the genetic background of the susceptible cultivar Nipponbare (NB), NB-Pib, NB-Pizt, NB-Pik and NB-Pita2; NILs bearing field resistance genes pi21 in the susceptible cultivar Aichiasahi (AA) AA-pi21, Kahei (KHR). The marker gene OsWRKY45 of salicylic acid (SA) signalling was upregulated in all tested cultivars. And, JAmyb (marker gene of jasmonic acid signalling) showed higher upregulation in the resistance lines with nucleotide-binding sites and leucine-rich repeat (NBS-LRR) R genes Pib, Pizt, Pik, Pita2 and Pikahei than in NB and KHS. SalT of abscisic acid (ABA) signalling may be involved in the R/Avr interaction, including Pizt, Pik, pi21 and Pikahei. However, SalT was shown to negatively regulate Pib/AvrPib interaction. OsPR1b and PBZ1 were differentially expressed and strongly activated at a later stage by 48 h post-inoculation. Interestingly, there was evidence that OsPR1b and PBZ1 played an important role in the pi21-mediated response. It was shown that OsRAR1 could be upregulated in the true resistance line NB-Pita2 and the field resistance line KHR, while OsSGT1 and OsHSP90 could be upregulated in all tested lines. The involvement of these genes illustrated the complexity of the downstream signalling pathways in the mediated resistance response of true/field resistance genes.

Keywords: field resistance; rice blast disease; signalling pathway; true resistance.

Grants and funding

This research was supported by grants from the National Science Foundation of China [grant number 31370306]; Project from Department of Human Resources and Social Security of China [grant number BZY12005]; the National Transgenic Research Project [grant number 2014ZX08009004B]; Hubei Provincial National Science Project [grant number 2013CFA098].