Gene array of PDL cells exposed to Osteogain in combination with a bone grafting material

Clin Oral Investig. 2016 Nov;20(8):2037-2043. doi: 10.1007/s00784-015-1702-2. Epub 2016 Jan 8.


Objectives: The aim of the present study was to investigate the effects of Osteogain, a new formulation of enamel matrix derivative (EMD) in combination with a grafting material on a wide variety of genes for cytokines, transcription factors and extracellular matrix proteins involved in osteoblast differentiation.

Materials and methods: Primary human periodontal ligament (PDL) cells were seeded on natural bone mineral (NBM) particles coated with Osteogain for 24 h and analyzed for regulated gene expression using a human osteogenesis gene super-array kit. Osteoblast-related genes include those transcribed during bone mineralization, ossification, bone metabolism, cell growth and differentiation as well as gene products representing extracellular matrix molecules, transcription factors and cell adhesion molecules.

Results: Osteogain significantly upregulated the expression of over 20 of the 100 genes examined including bone morphogenetic protein 2 (BMP2), TGFβ1, fibroblast growth factor (FGF), epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) as well as some of their associated receptors. Osteogain also promoted gene expression of a number of osteoblast differentiation markers including collagen1α2 and alkaline phosphatase as well as cell adhesion molecules including fibronectin and a variety of integrin binding proteins. Interestingly, Osteogain promoted calcitonin receptor 55-fold and also promoted annexin A5 gene expression over 12-fold.

Conclusion: The present study demonstrates that Osteogain is capable of either upregulating or downregulating the expression of a wide variety of genes including those for growth factors and cytokines when combined with a bone grafting material.

Clinical relevance: The results from the present study demonstrate the large and potent effect of addition of Osteogain in combination to a bone grafting material over a wide variety of genes supporting osteogenesis.

Keywords: Bone graft; Emdogain; Enamel matrix derivative; Enamel matrix proteins; Periodontal regeneration.

MeSH terms

  • Cell Adhesion / drug effects
  • Cell Adhesion / genetics
  • Cell Adhesion Molecules / genetics
  • Cell Differentiation / drug effects
  • Cell Differentiation / genetics
  • Cells, Cultured
  • Cytokines / genetics
  • Dental Enamel Proteins / pharmacology*
  • Extracellular Matrix Proteins / genetics
  • Gene Expression / drug effects
  • Humans
  • In Vitro Techniques
  • Minerals / pharmacology*
  • Osteoblasts / drug effects*
  • Periodontal Ligament / cytology*
  • Transcription Factors / genetics


  • Bio-Oss
  • Cell Adhesion Molecules
  • Cytokines
  • Dental Enamel Proteins
  • Extracellular Matrix Proteins
  • Minerals
  • Osteogain
  • Transcription Factors