Abstract
Full-length and deletion variants of the ets proto-oncogene and v-ets oncogene of E26 virus were expressed in E. coli using the previously described expression vectors pANH1, pJL6 and pJLA16. The recombinant proteins were expressed at greater than 5% of the total cellular proteins and were characterized by Western blot analysis using ets-specific antipeptide antibodies. A number of monoclonal antibodies were raised against p35, the expressed product of the partial human ets-2 proto-oncogene construct. These monoclonal antibodies are highly specific and recognize the p56 ets-2 product from several human cell lines.
MeSH terms
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Antibodies, Monoclonal / biosynthesis*
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Blotting, Western
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Escherichia coli / genetics*
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Genetic Vectors
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Humans
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Oncogenes*
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Proto-Oncogene Mas
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Proto-Oncogene Proteins / analysis
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins / immunology
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Proto-Oncogene Proteins c-ets
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Proto-Oncogenes*
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Retroviridae Proteins / analysis
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Retroviridae Proteins / genetics*
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Retroviridae Proteins, Oncogenic*
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Transcription Factors*
Substances
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Antibodies, Monoclonal
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MAS1 protein, human
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Proto-Oncogene Mas
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-ets
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Retroviridae Proteins
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Retroviridae Proteins, Oncogenic
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Transcription Factors
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oncogene proteins v-ets