Engineering Stem Cell Organoids

Cell Stem Cell. 2016 Jan 7;18(1):25-38. doi: 10.1016/j.stem.2015.12.005.


Organoid systems leverage the self-organizing properties of stem cells to create diverse multi-cellular tissue proxies. Most organoid models only represent single or partial components of a tissue, and it is often difficult to control the cell type, organization, and cell-cell/cell-matrix interactions within these systems. Herein, we discuss basic approaches to generate stem cell-based organoids, their advantages and limitations, and how bioengineering strategies can be used to steer the cell composition and their 3D organization within organoids to further enhance their utility in research and therapies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • Brain / metabolism
  • Cell Communication
  • Cell Culture Techniques*
  • Humans
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Intestinal Mucosa / metabolism
  • Microfluidics
  • Organogenesis
  • Organoids / cytology*
  • Regeneration
  • Stem Cells / cytology*
  • Tissue Engineering / methods


  • Intercellular Signaling Peptides and Proteins