Effect of Hesperidin With and Without a Calcium (Calcilock) Supplement on Bone Health in Postmenopausal Women

J Clin Endocrinol Metab. 2016 Mar;101(3):923-7. doi: 10.1210/jc.2015-3767. Epub 2016 Jan 11.


Context: Citrus fruits contain unique flavanones. One of the most abundant of the flavanones, hesperidin, has been shown to prevent bone loss in ovariectomized rats.

Objective: The objective of the study was to measure the effect of hesperidin with or without calcium supplementation on bone calcium retention in postmenopausal women.

Design: The study was a double-blind, placebo-controlled, randomized-order crossover design of 500 g hesperidin with or without 500 mg calcium supplement in 12 healthy postmenopausal women. Bone calcium retention was determined from urinary excretion of the rare isotope, (41)Ca, from bone.

Results: Calcium plus hesperidin, but not hesperidin alone, improved bone calcium retention by 5.5% (P < .04).

Conclusion: Calcium supplementation (Calcilock), in combination with hesperidin, is effective at preserving bone in postmenopausal women.

Trial registration: ClinicalTrials.gov NCT01881204.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Bone and Bones / drug effects*
  • Bone and Bones / metabolism*
  • Calcium / metabolism*
  • Calcium Radioisotopes / urine
  • Calcium, Dietary / administration & dosage*
  • Cross-Over Studies
  • Dietary Supplements
  • Double-Blind Method
  • Female
  • Hesperidin / administration & dosage*
  • Humans
  • Middle Aged
  • Osteoporosis, Postmenopausal / prevention & control
  • Placebos
  • Postmenopause*


  • Calcium Radioisotopes
  • Calcium, Dietary
  • Placebos
  • Hesperidin
  • Calcium

Associated data

  • ClinicalTrials.gov/NCT01881204