Cell type-specific bipolar cell input to ganglion cells in the mouse retina

Neuroscience. 2016 Mar 1;316:420-32. doi: 10.1016/j.neuroscience.2015.12.051. Epub 2016 Jan 2.

Abstract

Many distinct ganglion cell types, which are the output elements of the retina, were found to encode for specific features of a visual scene such as contrast, color information or movement. The detailed composition of retinal circuits leading to this tuning of retinal ganglion cells, however, is apart from some prominent examples, largely unknown. Here we aimed to investigate if ganglion cell types in the mouse retina receive selective input from specific bipolar cell types or if they sample their synaptic input non-selectively from all bipolar cell types stratifying within their dendritic tree. To address this question we took an anatomical approach and immunolabeled retinae of two transgenic mouse lines (GFP-O and JAM-B) with markers for ribbon synapses and type 2 bipolar cells. We morphologically identified all green fluorescent protein (GFP)-expressing ganglion cell types, which co-stratified with type 2 bipolar cells and assessed the total number of bipolar input synapses and the proportion of synapses deriving from type 2 bipolar cells. Only JAM-B ganglion cells received synaptic input preferentially from bipolar cell types other than type 2 bipolar cells whereas the other analyzed ganglion cell types sampled their bipolar input most likely from all bipolar cell terminals within their dendritic arbor.

Keywords: bipolar cell; connectivity; ganglion cell; mouse retina; selective input.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases
  • Animals
  • Calbindin 2 / metabolism
  • Co-Repressor Proteins
  • Computer Simulation
  • DNA-Binding Proteins / metabolism
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Mice
  • Mice, Transgenic
  • Microscopy, Confocal
  • Models, Neurological
  • Osteopontin / metabolism
  • Phosphoproteins / metabolism
  • Retina / cytology*
  • Retinal Bipolar Cells / metabolism
  • Retinal Bipolar Cells / physiology*
  • Retinal Bipolar Cells / ultrastructure
  • Retinal Ganglion Cells / classification*
  • Retinal Ganglion Cells / metabolism
  • Retinal Ganglion Cells / physiology*
  • Synaptotagmin II / metabolism
  • Thy-1 Antigens / genetics
  • Visual Pathways / physiology*

Substances

  • Calbindin 2
  • Co-Repressor Proteins
  • DNA-Binding Proteins
  • Luminescent Proteins
  • Phosphoproteins
  • Synaptotagmin II
  • Thy-1 Antigens
  • Osteopontin
  • Alcohol Oxidoreductases
  • Ctbp2 protein, mouse