2-Amino-2-deoxy-glucose conjugated cobalt ferrite magnetic nanoparticle (2DG-MNP) as a targeting agent for breast cancer cells

Environ Toxicol Pharmacol. 2016 Jan:41:272-8. doi: 10.1016/j.etap.2015.12.004. Epub 2015 Dec 17.

Abstract

In this study, 2-amino-2-deoxy-glucose (2DG) was conjugated to COOH modified cobalt ferrite magnetic nanoparticles (COOH-MNPs), which were designed to target tumor cells as a potential targetable drug/gene delivery agent for cancer treatment. According to our results, it is apparent that, 2DG labeled MNPs were internalized more efficiently than COOH-MNPs under the same conditions in all cell types (MDA-MB-231 and MCF-7 cancer and MCF-10A normal breast cells) (p<0.001). Moreover, the highest amount of uptake was observed in MDA-MB-231, followed by MCF-7 and normal MCF-10A cells for both MNPs. The apoptotic effects of 2DG-MNPs were further evaluated, and it was found that apoptosis was not induced at low concentrations of 2DG-MNPs in all cell types, whereas dramatic cell death was observed at higher concentrations. In addition, the gene expression levels of four drug-metabolizing enzymes, two Phase I (CYP1A1, CYP1B1) and two Phase II (GSTM3, GSTZ1) were also increased with the high concentrations of 2DG-MNPs.

Keywords: 2-Amino-2-deoxy-glucose; Breast cancer; Cobalt ferrite nanoparticles; Targeting therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis
  • Breast Neoplasms / drug therapy
  • Breast Neoplasms / metabolism
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Cobalt / chemistry*
  • Cobalt / pharmacology*
  • Dose-Response Relationship, Drug
  • Female
  • Ferric Compounds / chemistry*
  • Ferric Compounds / pharmacology*
  • Gene Expression Regulation, Neoplastic / drug effects
  • Glucosamine / chemistry*
  • Glucosamine / pharmacology*
  • Humans
  • MCF-7 Cells
  • Magnetite Nanoparticles / chemistry*

Substances

  • Ferric Compounds
  • Magnetite Nanoparticles
  • cobalt ferrite
  • Cobalt
  • Glucosamine