Perfluorocarbon attenuates inflammatory cytokines, oxidative stress and histopathologic changes in paraquat-induced acute lung injury in rats

Environ Toxicol Pharmacol. 2016 Mar:42:9-15. doi: 10.1016/j.etap.2015.12.002. Epub 2015 Dec 17.

Abstract

The effects of perfluorocarbon (PFC) on paraquat (PQ) induced acute lung injury (ALI) was evaluated among rats. Twenty four Wistar rats were divided into 4 groups: control group injected by saline physiologic 0.9%, PFC group injected by Perfluorocarbon, PQ group injected by PQ and PQ+PFC group injected by PFC one hour after receiving paraquat. Bronchoalveular fluid content, inflammatory cytokines, oxidative and histopathologic changes were measured after 72 h. The levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and transforming growth factor-β1(TGF-β1) in the PQ group were increased compared to either control or PFC groups, but their levels decreased in PQ+PFC group significantly (p<0.05). Also, histopathologic evaluation revealed an increase in malondialdehyde (MDA) and hydroxyproline (HP) in the PQ group but a decrease in PQ+PFC group significantly (p<0.01). PFC emulsion by its anti-inflammatory, anti-oxidative and anti-fibrotic properties can reduce the inflammatory and fibrotic alterations, pulmonary oedema, and pulmonary histopathologic changes created by PQ.

Keywords: Histopathology; Inflammation; Lung; Malondealdehyde; Paraquat; Perfluorocarbon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Lung Injury
  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Cytokines / metabolism*
  • Fluorocarbons / pharmacology*
  • Interleukin-6
  • Male
  • Oxidative Stress / drug effects
  • Paraquat
  • Rats
  • Rats, Wistar
  • Transforming Growth Factor beta1 / metabolism
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Anti-Inflammatory Agents
  • Cytokines
  • Fluorocarbons
  • Interleukin-6
  • Transforming Growth Factor beta1
  • Tumor Necrosis Factor-alpha
  • Paraquat