Galectins in channel catfish, Ictalurus punctatus: Characterization and expression profiling in mucosal tissues

Fish Shellfish Immunol. 2016 Feb;49:324-35. doi: 10.1016/j.fsi.2016.01.005. Epub 2016 Jan 6.

Abstract

Galectins, a family of β-galactoside-binding lectins with conserved CRDs, which can recognize the glycans on the surface of viruses, bacteria and protozoan parasites, are emerging as key players in many important pathological processes, including acute and chronic inflammatory diseases, autoimmunity and apoptosis. Although galectins have attracted great interest in mammals, they are still poorly-characterized in teleost. Previously, several studies have reported their high expression levels in mucosal tissues before and post infection. Given the important roles for galectins in mucosal immunity, therefore, we characterized the galectin gene family and profiled family member expression after challenge with two different Gram-negative bacterial pathogens. Here, twelve galectins genes were captured in channel catfish (Ictalurus punctatus), and phylogenetic analysis showed the strongest relationship to zebrafish and salmon, which is consistent with their phylogenetic relationships. Furthermore, the galectin genes were widely expressed in catfish tissues, while most of the galectin genes were strongly expressed in mucosal tissues (skin, gill and intestine). In addition, the expression profiles of galectins after bacterial infection varied depending on both pathogen and tissue type, suggesting that galectins may exert disparate functions or exhibit distinct tissue-selective roles in the host immune response to bacterial pathogens. Further studies are needed, however, to expand functional characterization and examine whether galectins may also play additional physiological roles in catfish immunity.

Keywords: Catfish; Galectin; Infection; Mucosal immunity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Edwardsiella ictaluri / physiology
  • Enterobacteriaceae Infections / immunology
  • Enterobacteriaceae Infections / microbiology
  • Enterobacteriaceae Infections / veterinary*
  • Fish Diseases / immunology*
  • Fish Diseases / microbiology
  • Flavobacteriaceae Infections / immunology
  • Flavobacteriaceae Infections / microbiology
  • Flavobacteriaceae Infections / veterinary*
  • Flavobacterium / physiology
  • Galectins / genetics*
  • Galectins / metabolism
  • Gene Expression Profiling
  • Gene Expression Regulation*
  • Ictaluridae* / classification
  • Ictaluridae* / genetics
  • Ictaluridae* / metabolism
  • Immunity, Mucosal / immunology
  • Molecular Sequence Data
  • Mucous Membrane / immunology*
  • Mucous Membrane / microbiology
  • Phylogeny
  • Sequence Alignment / veterinary
  • Sequence Analysis, Protein / veterinary

Substances

  • Galectins