Antioxidant, Anti-inflammatory, and Antiulcer Potential of Manuka Honey against Gastric Ulcer in Rats

Oxid Med Cell Longev. 2016;2016:3643824. doi: 10.1155/2016/3643824. Epub 2015 Dec 7.

Abstract

Gastric ulcers are among the most common diseases affecting humans. This study aimed at investigating the gastroprotective effects of manuka honey against ethanol-induced gastric ulcers in rats. The mechanism by which honey exerts its antiulcer potential was elucidated. Four groups of rats were used: control, ethanol (ulcer), omeprazole, and manuka honey. Stomachs were examined macroscopically for hemorrhagic lesions in the glandular mucosa, histopathological changes, and glycoprotein detection. The effects of oxidative stress were investigated using the following indicators: gastric mucosal nitric oxide (NO), reduced glutathione (GSH), lipid peroxide (MDA, measured as malondialdehyde) glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase. Plasma tumour necrosis factor-α, interleukin-1β, and IL-6 were also measured. Manuka honey significantly decreased the ulcer index, completely protected the mucosa from lesions, and preserved gastric mucosal glycoprotein. It significantly increased gastric mucosal levels of NO, GSH, GPx, and SOD. Manuka honey also decreased gastric mucosal MDA and plasma TNF-α, IL-1β, and IL-6 concentrations. In conclusion, manuka honey likely exerted its antiulcer, effect by keeping enzymatic (GPx and SOD) and nonenzymatic (GSH and NO) antioxidants as well as inflammatory cytokines (TNF-α, IL-1β, and IL-6) in a reduced form, inhibited lipid peroxidation (MDA), and preserved mucous glycoproteins levels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Anti-Inflammatory Agents / therapeutic use*
  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use*
  • Blood Glucose / metabolism
  • Catalase / metabolism
  • Cholesterol / blood
  • Disease Models, Animal
  • Drinking Behavior / drug effects
  • Ethanol
  • Feeding Behavior / drug effects
  • Glutathione / metabolism
  • Glutathione Peroxidase / metabolism
  • Glycoproteins / metabolism
  • Honey*
  • Interleukin-1beta / blood
  • Interleukin-6 / blood
  • Male
  • Malondialdehyde / metabolism
  • Nitric Oxide / metabolism
  • Rats
  • Staining and Labeling
  • Stomach Ulcer / drug therapy*
  • Stomach Ulcer / pathology
  • Superoxide Dismutase / metabolism
  • Triglycerides / blood
  • Tumor Necrosis Factor-alpha / blood
  • Weight Gain / drug effects

Substances

  • Anti-Inflammatory Agents
  • Antioxidants
  • Blood Glucose
  • Glycoproteins
  • Interleukin-1beta
  • Interleukin-6
  • Triglycerides
  • Tumor Necrosis Factor-alpha
  • Nitric Oxide
  • Ethanol
  • Malondialdehyde
  • Cholesterol
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione