Amphotericin B-silver hybrid nanoparticles: synthesis, properties and antifungal activity

Nanomedicine. 2016 May;12(4):1095-1103. doi: 10.1016/j.nano.2015.12.378. Epub 2016 Jan 7.


High antifungal activity is reported, in comparison with commercially available products, of a novel hybrid system based on silver nanoparticles synthesized using a popular antifungal macrocyclic polyene amphotericin B (AmB) acting both as a reducing and stabilizing/capping agent. The synthesis reaction proceeds in an alkaline environment which prevents aggregation of AmB itself and promotes nanoparticle formation. The innovative approach produces monodisperse (PDI=0.05), AmB-coated silver nanoparticles (AmB-AgNPs) with the diameter ~7nm. The products were characterized using imaging (electron microscopy) and spectroscopic (UV-vis and infrared absorption, dynamic light scattering and Raman scattering) methods. The nanoparticles were tested against Candida albicans, Aspergillus niger and Fusarium culmorum species. For cytotoxicity studies CCD-841CoTr and THP-1 cell lines were used. Particularly high antifungal activity of AmB-AgNPs is interpreted as the result of synergy between the antifungal activity of amphotericin B and silver antimicrobial properties (Ag(+) ions release).

From the clinical editor: Amphotericin B (AmB) is a common agent used for the treatment against severe fungal infections. In this article, the authors described a new approach in using a combination of AmB and silver nanoparticles, in which the silver nanoparticles were synthesized and stabilized by AmB. Experimental data confirmed synergistic antifungal effects between amphotericin B and silver. This novel synthesis process could potentially be important in future drug development and fabrication.

Keywords: Amphotericin B; Antifungal activity; Bioconjugate; Nanoparticle synthesis; Silver nanoparticles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amphotericin B / chemical synthesis
  • Amphotericin B / chemistry
  • Amphotericin B / pharmacology*
  • Antifungal Agents / chemical synthesis
  • Antifungal Agents / chemistry
  • Antifungal Agents / pharmacology*
  • Aspergillus niger / drug effects
  • Aspergillus niger / pathogenicity
  • Candida albicans / drug effects
  • Candida albicans / pathogenicity
  • Drug Delivery Systems
  • Fusarium / drug effects
  • Fusarium / pathogenicity
  • Humans
  • Mycoses / drug therapy*
  • Mycoses / microbiology
  • Nanoparticles / administration & dosage*
  • Nanoparticles / chemistry
  • Silver / chemistry
  • Silver / pharmacology


  • Antifungal Agents
  • Silver
  • Amphotericin B