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. 2016 May;107(3):274-86.
doi: 10.1093/jhered/esw004. Epub 2016 Jan 16.

Genetic Diversity of the Two Commercial Tetraploid Cotton Species in the Gossypium Diversity Reference Set

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Free PMC article

Genetic Diversity of the Two Commercial Tetraploid Cotton Species in the Gossypium Diversity Reference Set

Lori L Hinze et al. J Hered. .
Free PMC article

Erratum in

  • Erratum.
    J Hered. 2016 Sep;107(5):478. doi: 10.1093/jhered/esw022. Epub 2016 May 20. J Hered. 2016. PMID: 27208147 Free PMC article. No abstract available.

Abstract

A diversity reference set has been constructed for the Gossypium accessions in the US National Cotton Germplasm Collection to facilitate more extensive evaluation and utilization of accessions held in the Collection. A set of 105 mapped simple sequence repeat markers was used to study the allelic diversity of 1933 tetraploid Gossypium accessions representative of the range of diversity of the improved and wild accessions of G. hirsutum and G. barbadense. The reference set contained 410 G. barbadense accessions and 1523 G. hirsutum accessions. Observed numbers of polymorphic and private bands indicated a greater diversity in G. hirsutum as compared to G. barbadense as well as in wild-type accessions as compared to improved accessions in both species. The markers clearly differentiated the 2 species. Patterns of diversity within species were observed but not clearly delineated, with much overlap occurring between races and regions of origin for wild accessions and between historical and geographic breeding pools for cultivated accessions. Although the percentage of accessions showing introgression was higher among wild accessions than cultivars in both species, the average level of introgression within individual accessions, as indicated by species-specific bands, was much higher in wild accessions of G. hirsutum than in wild accessions of G. barbadense. The average level of introgression within individual accessions was higher in improved G. barbadense cultivars than in G. hirsutum cultivars. This molecular characterization reveals the levels and distributions of genetic diversity that will allow for better exploration and utilization of cotton genetic resources.

Keywords: Gossypium barbadense; Gossypium hirsutum; germplasm collection; introgression; population structure.

Figures

Figure 1.
Figure 1.
Principal coordinate plot of the first 2 coordinate axes showing patterns of separation between G. hirsutum and G. barbadense that reflect 31.2% of the total variation. Thirty-six putative misclassified accessions were removed prior to analyses.
Figure 2.
Figure 2.
Principal coordinate plots showing patterns of separation between wild and improved accessions within (A) G. hirsutum explaining 23.13% of the variation and (B) G. barbadense explaining 16.11% of the variation. Accessions which could not be classified into either category are labeled “n/a”.
Figure 3.
Figure 3.
Analysis of population structure for improved Gossypium accessions. Principal coordinate plots showing patterns of separation within (A) G. barbadense explaining 18.32% of the variation and (B) G. hirsutum from the United States explaining 12.42% of the variation. Accessions which could not be classified into a category are labelled “n/a”. (C) Results of improved US G. hirsutum genetic analysis without prior population assignment. Each accession’s genome is symbolized by a thin vertical line divided into colored segments representing the proportion of empirically defined genetic pools (K value equal to 5). Genetic pools are Western (red), Midsouth (green), Plains (blue), Eastern (yellow), and n/a (pink).
Figure 4.
Figure 4.
Principal coordinate plots showing patterns of separation between categories of wild accessions within (A) G. barbadense regions explaining 12.01% of the variation and (B) G. hirsutum races explaining 19.60% of the variation. Accessions which could not be classified into a category are labeled “n/a”.

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