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Effects of Collagen Grafting on Cell Behaviors in BCP Scaffold With Interconnected Pore Structure

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Effects of Collagen Grafting on Cell Behaviors in BCP Scaffold With Interconnected Pore Structure

Dong-Jun Yang et al. Biomater Res.

Abstract

Background: This study was to investigate the effect of collagen grafted porous biphasic calcium phosphate (BCP) on cell attachment, proliferation, and differentiation. Porous BCP scaffolds with interconnected micropore structure were prepared with were prepared and then grafted with a collagen type I. The hydroxyapatite (HA) and β-tricalcium phosphate (TCP) ratio of the TCP scaffolds was about 60/40 and the collagen was crosslinked on the TCP scaffold surface (collagen-TCP).

Results: The sintered BCP scaffolds showed fully interconnected micropore structures with submicron-sized grains. The collagen crosslinking in the scaffolds was conducted using the the N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (NHS) crosslinking method. The cell proliferation of collagen-BCP scaffolds showed a similar result to that of the BCP scaffolds. However, osteoblastic differentiation and cell attachment increased in the collagen-BCP scaffolds.

Conclusions: Collagen-BCP scaffold improved the cell attachment ability in early phase and osteoblastic differentiation.

Keywords: Biphasic calcium phosphate (BCP); Collagen; Hydroxyapatite (HA); Tricalcium phosphate (TCP).

Figures

Fig. 1
Fig. 1
X-ray diffraction of BCP scaffolds and collagen/ BCP scaffolds
Fig. 2
Fig. 2
Scanning electron microscope image of BCP scaffolds and collagen/ BCP scaffolds. SEM images of BCP scaffolds (a, b, c) and collagen/ BCP scaffolds (d, e, f) at magnifications of × 1000 (a, d), ×10000 (b, e) and × 20000 (c, f)
Fig. 3
Fig. 3
X-ray photoelectron spectroscopy spectra of BCP scaffolds and collagen/ BCP scaffolds. XPS of BCP scaffolds (a) and collagen/ BCP scaffolds (b)
Fig. 4
Fig. 4
Coomassie brilliant blue staining of collagen/ BCP scaffolds
Fig. 5
Fig. 5
Cell morphology of MC3T3-E1 on BCP scaffolds and collagen/BCP scaffolds. SEM images of MC3T3-E1 cultured on BCP scaffolds (a, c) and collagen-BCP scaffolds (b, d) for 24 h at magnifications of × 500 (a, b) and × 10000 (c, d)
Fig. 6
Fig. 6
Cell proliferation of MC3T3-E1 cultured on BCP scaffolds and collagen/ BCP scaffolds. Proliferation of MC3T3-E1 cells were determined by MTT assay (a) and cell counting (b). Data are expressed as the mean ± SD (n = 3)
Fig. 7
Fig. 7
ALP staining of differentiating MC3T3-E1 cultured on BCP scaffolds and collagen/BCP scaffolds. MC3T3-E1 cells were cultured in osteogenic media, and then ALP staining was performed. ALP staining image of BCP scaffolds (a) and collagen/BCP scaffolds (b)

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