A study of the conformation of 5S RNA by 31P NMR

Nucleic Acids Res. 1989 Sep 25;17(18):7295-302. doi: 10.1093/nar/17.18.7295.

Abstract

Only a small number of resolved, single phosphorous, phosphodiester resonances are observed in the 31P spectrum of the 5S rRNA from E. coli. Its spectrum is much simpler than that of a tRNA (Gueron, M. and Shulman, R.G. (1975) Proc. Natl. Acad. Sci. 72, 3482-3484), which suggests that 5S RNA does not have a tightly folded, tRNA-like, tertiary structure. The resolved resonances in the 5S spectrum originate in loops D and E, near bases 88 and 76, respectively.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cations, Divalent
  • Escherichia coli / ultrastructure
  • Hydrogen Bonding
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • RNA, Ribosomal
  • RNA, Ribosomal, 5S / ultrastructure*

Substances

  • Cations, Divalent
  • RNA, Ribosomal
  • RNA, Ribosomal, 5S