Induction of Tolerance Against the Arthritogenic Antigen With type-II Collagen Peptide-Linked Soluble MHC Class II Molecules

BMB Rep. 2016 Jun;49(6):331-6. doi: 10.5483/bmbrep.2016.49.6.207.

Abstract

In murine collagen-induced arthritis (CIA), self-reactive T cells can recognize peptide antigens derived from type-II collagen (CII). Activation of T cells is an important mediator of autoimmune diseases. Thus, T cells have become a focal point of study to treat autoimmune diseases. In this study, we evaluated the efficacy of recombinant MHC class II molecules in the regulation of antigen-specific T cells by using a self peptide derived from CII (CII260-274; IAGFKGEQGPKGEPG) linked to mouse I-A(q) in a murine CIA model. We found that recombinant I-A(q)/CII260-274 molecules could be recognized by CII-specific T cells and inhibit the same T cells in vitro. Furthermore, the development of CIA in mice was successfully prevented by in vivo injection of recombinant I-A(q)/CII260-274 molecules. Thus, treatment with recombinant soluble MHC class II molecules in complex with an immunodominant self-peptide might offer a potential therapeutic for chronic inflammation in autoimmune disease such as rheumatoid arthritis. [BMB Reports 2016; 49(6): 331-336].

Publication types

  • News

MeSH terms

  • Animals
  • Antigens / immunology*
  • Arthritis, Experimental / immunology*
  • Arthritis, Experimental / pathology
  • CD4-Positive T-Lymphocytes / immunology
  • Collagen Type II / immunology*
  • Cytokines / metabolism
  • Histocompatibility Antigens Class II / immunology*
  • Immune Tolerance*
  • Inflammation Mediators / metabolism
  • Lymphocyte Count
  • Mice
  • Peptides / immunology*
  • Recombinant Proteins / immunology
  • Solubility

Substances

  • Antigens
  • Collagen Type II
  • Cytokines
  • Histocompatibility Antigens Class II
  • Inflammation Mediators
  • Peptides
  • Recombinant Proteins