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. 2016 May;48(5):1253-62.
doi: 10.1007/s00726-016-2174-7. Epub 2016 Jan 18.

Aspergillus Niger PA2: A Novel Strain for Extracellular Biotransformation of L-tyrosine Into L-DOPA

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Aspergillus Niger PA2: A Novel Strain for Extracellular Biotransformation of L-tyrosine Into L-DOPA

Pragati Agarwal et al. Amino Acids. .

Abstract

L-DOPA (3,4-dihydroxyphenyl-L-alanine), an amino acid derivative is the most widely used drug of choice for the treatment of Parkinson's disease and other neurologic injuries. The present study deals with the elevated biochemical transformation of L-tyrosine to L-DOPA by Aspergillus niger PA2, a potent tyrosinase producer, isolated from decomposed food wastes. This appears to be the first report on A. niger as a notable extracellular tyrosinase producer. The extracellular tyrosinase activity produced remarkably higher levels of L-DOPA, i.e. 2.44 mg mL(-1) when the media was supplemented with 5 mg mL(-1) L-tyrosine. The optimum pH for tyrosinase production was 6.0, with the maximal L-DOPA production at the same pH. The product thus produced was analyzed by thin-layer chromatography, UV spectroscopy, high-performance liquid chromatography and Fourier transform infrared spectroscopy, that had denoted this to be L-DOPA. Kinetic parameters viz. Y p/s, Q s and Q p had further indicated the notable levels of production. Thus, Aspergillus niger PA2 could be a promising resource and may be further exploited for large-scale production of L-DOPA.

Keywords: Aspergillus niger; Biotransformation; L-DOPA; Tyrosinase.

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