Maggot debridement therapy promotes diabetic foot wound healing by up-regulating endothelial cell activity

J Diabetes Complications. 2016 Mar;30(2):318-22. doi: 10.1016/j.jdiacomp.2015.11.009. Epub 2015 Nov 17.


To determine the role of maggot debridement therapy (MDT) on diabetic foot wound healing, we compared growth related factors in wounds before and after treatment. Furthermore, we utilized human umbilical vein endothelial cells (HUVECs) to explore responses to maggot excretions/secretions on markers of angiogenesis and proliferation. The results showed that there was neo-granulation and angiogenesis in diabetic foot wounds after MDT. Moreover, significant elevation in CD34 and CD68 levels was also observed in treated wounds. In vitro, ES increased HUVEC proliferation, improved tube formation, and increased expression of vascular endothelial growth factor receptor 2 in a dose dependent manner. These results demonstrate that MDT and maggot ES can promote diabetic foot wound healing by up-regulating endothelial cell activity.

Keywords: Angiogenesis; Diabetic foot ulcers; HUVEC; Maggot debridement therapy; Wound healing.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Animals
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Culture Media, Conditioned / metabolism
  • Culture Media, Conditioned / pharmacology
  • Debridement / methods*
  • Diabetic Foot / pathology
  • Diabetic Foot / physiopathology
  • Diabetic Foot / therapy*
  • Endothelial Cells / drug effects
  • Endothelial Cells / physiology*
  • Human Umbilical Vein Endothelial Cells / drug effects
  • Human Umbilical Vein Endothelial Cells / physiology
  • Humans
  • Larva / metabolism
  • Neovascularization, Physiologic / drug effects
  • Neovascularization, Physiologic / physiology
  • Up-Regulation
  • Wound Healing / drug effects
  • Wound Healing / physiology*


  • Culture Media, Conditioned