HPV infection-associated anogenital cyto-colpo-histological findings and molecular typing in HIV-positive women

Genet Mol Res. 2015 Dec 21;14(4):17630-40. doi: 10.4238/2015.December.21.36.

Abstract

HIV and human papillomavirus (HPV) coinfection is increasing, especially in the anal canal (AC) and cervico-vaginal regions. We identified anal epithelium abnormalities related to high-risk HPV (HR-HPV) lesions in the lower genital tracts (LGTs) of HIV-positive women, described the HPV genotypes identified, and assessed the expression of E6/E7 oncogenes in coinfected patients. Ninety-eight women were enrolled in groups combining HIV status and presence or absence of HPV in the LGT. Anal and cervical smears were collected for cytology and HR-HPV assays using Cobas(®) and/or PapilloCheck(®). Samples with highly oncogenic HPV genotypes were confirmed by NucliSENS EasyQ(®). Forty-two HIV-positive (25-52; mean age 39.5) and 56 HIV-negative (18-58; mean age 35.7) patients were included. E2 and C1 groups presented AC alterations (P = 0.002); altered images for high-resolution anoscopy were higher in E1 and C2 (P < 0.001). Of the 29 women with alterations, 41.38% were HIV-negative and 58.62% were HIV-positive (P < 0.001). HIV-positive patients accounted for 29% of the anal high-grade squamous intraepithelial lesions (P = 0.015). The Cobas(®) positive result frequency was higher in three AC groups than in the other groups. There was variation in the number of HPV types in the cervico-vaginal samples among the study groups (P < 0.001). Anal cytology and anoscopy showed more altered findings in HIV-positive patients with HPV in the LGT. HR-HPV anal infections by various genotypes are common and are associated with cervical infections in HIV-positive patients. E6/E7 expression is apparently more common in the AC of HIV-positive women.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Anal Canal / pathology
  • Anal Canal / virology
  • Coinfection / complications
  • Coinfection / pathology
  • Coinfection / virology*
  • Female
  • Genotype
  • HIV / genetics
  • HIV / isolation & purification
  • HIV / pathogenicity
  • HIV Infections / complications
  • HIV Infections / genetics
  • HIV Infections / pathology
  • HIV Infections / virology*
  • Humans
  • Middle Aged
  • Oncogene Proteins, Viral / biosynthesis
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification
  • Papillomaviridae / pathogenicity
  • Papillomavirus Infections / complications
  • Papillomavirus Infections / genetics
  • Papillomavirus Infections / pathology
  • Papillomavirus Infections / virology*
  • RNA, Messenger / biosynthesis
  • Repressor Proteins / biosynthesis
  • Reproductive Tract Infections / complications
  • Reproductive Tract Infections / genetics
  • Reproductive Tract Infections / pathology
  • Reproductive Tract Infections / virology*
  • Vaginal Smears

Substances

  • E6 protein, Human papillomavirus type 16
  • Oncogene Proteins, Viral
  • RNA, Messenger
  • Repressor Proteins