First Description of Two Sequence Type 2 Acinetobacter baumannii Isolates Carrying OXA-23 Carbapenemase in Pagellus acarne Fished from the Mediterranean Sea near Bejaia, Algeria

Soumia Brahmi; Abdelaziz Touati; Axelle Cadière; Nassima Djahmi; Alix Pantel; Albert Sotto; Jean-Philippe Lavigne; Catherine Dunyach-Remy

doi:10.1128/AAC.02384-15

First Description of Two Sequence Type 2 Acinetobacter baumannii Isolates Carrying OXA-23 Carbapenemase in Pagellus acarne Fished from the Mediterranean Sea near Bejaia, Algeria

Antimicrob Agents Chemother. 2016 Mar 25;60(4):2513-5. doi: 10.1128/AAC.02384-15. Print 2016 Apr.

Authors

Soumia Brahmi¹, Abdelaziz Touati², Axelle Cadière³, Nassima Djahmi⁴, Alix Pantel⁵, Albert Sotto⁶, Jean-Philippe Lavigne⁷, Catherine Dunyach-Remy⁵

Affiliations

¹ INSERM, U1047, University of Montpellier, Nîmes, France Laboratory of Microbial Ecology, Department of Microbiology, A/MIRA University, Bejaia, Algeria.
² Laboratory of Microbial Ecology, Department of Microbiology, A/MIRA University, Bejaia, Algeria.
³ Chrome Unit, EA7352, University of Nîmes, Nîmes, France.
⁴ Department of Microbiology, University Hospital Ibn Rochd, Annaba, Algeria.
⁵ INSERM, U1047, University of Montpellier, Nîmes, France Department of Microbiology, Caremeau University Hospital, Nîmes, France.
⁶ INSERM, U1047, University of Montpellier, Nîmes, France.
⁷ INSERM, U1047, University of Montpellier, Nîmes, France Department of Microbiology, Caremeau University Hospital, Nîmes, France jean.philippe.lavigne@chu-nimes.fr.

Abstract

To determine the occurrence of carbapenem-resistantAcinetobacter baumanniiin fish fished from the Mediterranean Sea near the Bejaia coast (Algeria), we studied 300 gills and gut samples that had been randomly and prospectively collected during 1 year. After screening on selective agar media, using PCR arrays and whole-genome sequencing, we identified for the first time two OXA-23-producingA. baumanniistrains belonging to the widespread sequence type 2 (ST2)/international clone II and harboring aminoglycoside-modifying enzymes [aac(6')-Ib andaac(3')-I genes].

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetyltransferases / genetics*
Acetyltransferases / metabolism
Acinetobacter Infections / epidemiology
Acinetobacter Infections / microbiology
Acinetobacter Infections / veterinary*
Acinetobacter baumannii / classification
Acinetobacter baumannii / drug effects
Acinetobacter baumannii / genetics*
Acinetobacter baumannii / isolation & purification
Algeria / epidemiology
Animals
Anti-Bacterial Agents / pharmacology
Carbapenems / pharmacology
Fish Diseases / epidemiology
Fish Diseases / microbiology
Fish Proteins / genetics*
Fish Proteins / metabolism
Gastrointestinal Tract / microbiology
Gene Expression
Genotype
Gills / microbiology
Mediterranean Sea / epidemiology
Perciformes
Phylogeny
beta-Lactam Resistance / genetics*
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Anti-Bacterial Agents
Carbapenems
Fish Proteins
Acetyltransferases
aminoglycoside acetyltransferase
beta-lactamase OXA-23
beta-Lactamases

Grants and funding

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.