Hydrogen Exchange Mass Spectrometry

Abstract

Hydrogen exchange (HX) methods can reveal much about the structure, energetics, and dynamics of proteins. The addition of mass spectrometry (MS) to an earlier fragmentation-separation HX analysis now extends HX studies to larger proteins at high structural resolution and can provide information not available before. This chapter discusses experimental aspects of HX labeling, especially with respect to the use of MS and the analysis of MS data.

Keywords: Epitope mapping; HDX-MS; HX-MS; Hydrogen exchange; Hydrogen–deuterium exchange; Mass spectrometry; Protein dynamics; Protein folding; Protein stability; Protein structure.