Abca7 deletion does not affect adult neurogenesis in the mouse

Hongyun Li; Tim Karl; Brett Garner

doi:10.1042/BSR20150308

Abca7 deletion does not affect adult neurogenesis in the mouse

Biosci Rep. 2016 Jan 20;36(2):e00308. doi: 10.1042/BSR20150308.

Authors

Hongyun Li¹, Tim Karl², Brett Garner³

Affiliations

¹ Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW 2522, Australia School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia.
² Neuroscience Research Australia, Randwick, NSW 2031, Australia School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia Schizophrenia Research Institute, Randwick, NSW 2031, Australia.
³ Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW 2522, Australia School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia brettg@uow.edu.au.

Abstract

ATP-binding cassette transporter A7 (ABCA7) is highly expressed in the brain. Recent genome-wide association studies (GWAS) have identified ABCA7 single nucleotide polymorphisms (SNPs) that increase Alzheimer's disease (AD) risk, however, the mechanisms by which ABCA7 may control AD risk remain to be fully elucidated. Based on previous research suggesting that certain ABC transporters may play a role in the regulation of neurogenesis, we conducted a study of cell proliferation and neurogenic potential using cellular bromodeoxyuridine (BrdU) incorporation and doublecortin (DCX) immunostaining in adult Abca7 deficient mice and wild-type-like (WT) littermates. In the present study counting of BrdU-positive and DCX-positive cells in an established adult neurogenesis site in the dentate gyrus (DG) indicated there were no significant differences when WT and Abca7 deficient mice were compared. We also measured the area occupied by immunohistochemical staining for BrdU and DCX in the DG and the subventricular zone (SVZ) of the same mice and this confirmed that ABCA7 does not play a significant role in the regulation of cell proliferation or neurogenesis in the adult mouse.

Keywords: ABCA7; Alzheimer's disease; bromodeoxyuridine; dentate gyrus; doublecortin; neurogenesis; subventricular zone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ATP-Binding Cassette Transporters / deficiency*
ATP-Binding Cassette Transporters / metabolism
Alzheimer Disease / genetics
Alzheimer Disease / metabolism*
Alzheimer Disease / pathology
Animals
Cell Proliferation*
Dentate Gyrus / metabolism*
Dentate Gyrus / pathology
Doublecortin Protein
Lateral Ventricles / metabolism*
Lateral Ventricles / pathology
Mice
Mice, Knockout
Neurogenesis*
Polymorphism, Single Nucleotide

Substances

ATP-Binding Cassette Transporters
Abca7 protein, mouse
Dcx protein, mouse
Doublecortin Protein