Chemical characterization of Pleurotus eryngii polysaccharide and its tumor-inhibitory effects against human hepatoblastoma HepG-2 cells

Carbohydr Polym. 2016 Mar 15:138:123-33. doi: 10.1016/j.carbpol.2015.11.051. Epub 2015 Dec 2.

Abstract

This study was designed to investigate the chemical characterization and antitumor effects of Pleurotus eryngii polysaccharides (PEP). The crude PEP was fractionated into two fractions, namely PEP-1 and PEP-2. HPLC analysis showed that PEP-1 and PEP-2 were heteropolysaccharides mainly composed of glucose with the average molecular weights of 2.54×10(4)Da (PEP-1) and 4.63×10(5)Da (PEP-2), respectively. High molecular mass PEP-2 was shown to exhibit stronger growth inhibition against human hepatoblastoma HepG-2 cells in comparison with PEP-1. Flow cytometric analysis showed that PEP-2 exerted a stimulatory effect on apoptosis of HepG-2 cells, and induced the cell-cycle arrest at the S-phase, with the observation of intracellular ROS production. These findings suggest that the polysaccharides, especially PEP-2, are very important nutritional ingredients responsible for the anticancer health benefits of P. eryngii.

Keywords: Anti-proliferation; Apoptosis; Cell cycle; Pleurotus eryngii; Polysaccharide; Purification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / analysis
  • Antineoplastic Agents / chemistry*
  • Antineoplastic Agents / toxicity
  • Apoptosis / drug effects
  • Caspase 3 / metabolism
  • Caspase 9 / metabolism
  • Chromatography, High Pressure Liquid
  • Hep G2 Cells
  • Hepatoblastoma / metabolism
  • Hepatoblastoma / pathology
  • Humans
  • Molecular Weight
  • Pleurotus / metabolism*
  • Polysaccharides / analysis
  • Polysaccharides / chemistry*
  • Polysaccharides / toxicity
  • Reactive Oxygen Species / metabolism
  • S Phase Cell Cycle Checkpoints / drug effects

Substances

  • Antineoplastic Agents
  • Polysaccharides
  • Reactive Oxygen Species
  • Caspase 3
  • Caspase 9