On-chip, aptamer-based sandwich assay for detection of glycated hemoglobins via magnetic beads

Jinglun Li; Ko-Wei Chang; Chih-Hung Wang; Ching-Hsuan Yang; Shu-Chu Shiesh; Gwo-Bin Lee

doi:10.1016/j.bios.2016.01.029

On-chip, aptamer-based sandwich assay for detection of glycated hemoglobins via magnetic beads

Biosens Bioelectron. 2016 May 15:79:887-93. doi: 10.1016/j.bios.2016.01.029. Epub 2016 Jan 12.

Authors

Jinglun Li¹, Ko-Wei Chang¹, Chih-Hung Wang¹, Ching-Hsuan Yang², Shu-Chu Shiesh³, Gwo-Bin Lee⁴

Affiliations

¹ Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
² Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
³ Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, Tainan, Taiwan. Electronic address: hsieh@mail.ncku.edu.tw.
⁴ Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan; Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan; Institute of NanoEngineering and Microsystems, National Tsing Hua University, Hsinchu, Taiwan. Electronic address: gwobin@pme.nthu.edu.tw.

PMID: 26797251
DOI: 10.1016/j.bios.2016.01.029

Abstract

Diabetes can be diagnosed and monitored by measurement of the cutoff ratio between glycated hemoglobins (HbA1c) and total hemoglobin (Hb), which does not require a fasting blood sample and is less influenced by biological variations. In this study, we combined the advantages of the microfluidic system and the selected low-cost, stable and specific aptamers and developed an integrated, aptamer-based microfluidic system for automatic glycated hemoglobin measurements. The detection process of human whole blood can be totally automated in this integrated microfluidic system. According to the experimental results, when compared to conventional bench-top manual assays, reagent consumption was significantly reduced by 75%, and the analysis time was reduced from 3.5h to 30 min. Besides, the novelty in this research also lies in the simultaneously performed two parallel assays for detection of Hb and HbA1c in a single chip. Therefore, this sensitive and low-cost aptamer-based microfluidic system may become a promising tool for point-of -care diagnosis of diabetes.

Keywords: Aptamer; Diabetes; Microfluidics; On-chip diagnostics.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry*
Base Sequence
Biosensing Techniques / instrumentation*
Biosensing Techniques / methods
Equipment Design
Glycated Hemoglobin / analysis*
Humans
Lab-On-A-Chip Devices*
Limit of Detection
Magnets / chemistry
Molecular Sequence Data

Substances

Aptamers, Nucleotide
Glycated Hemoglobin A