DELLA proteins physically interact with CONSTANS to regulate flowering under long days in Arabidopsis

FEBS Lett. 2016 Feb;590(4):541-9. doi: 10.1002/1873-3468.12076. Epub 2016 Feb 7.


Proper timing of flowering is essential for reproduction of plants. Although it is well known that both light and gibberellin (GA) signaling play critical roles in promoting flowering in Arabidopsis thaliana, whether and how they are integrated to regulate flowering remain largely unknown. Here, we show through biochemical studies that DELLA proteins physically interact with CONSTANS (CO). Furthermore, the interaction of CO with NF-YB2 is inhibited by the DELLA protein, RGA. Our findings suggest that regulation of flowering by GA signaling in leaves under long days is mediated, at least in part, through repression of DELLA proteins on CO, providing a molecular link between DELLA proteins, key components in GA signaling pathway, and CO, a critical flowering activator in photoperiod signaling pathway.

Keywords: CONSTANS; DELLA proteins; Nuclear Factor Y; flowering; photoperiodic.

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / growth & development*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • CCAAT-Binding Factor / genetics
  • CCAAT-Binding Factor / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Flowers / genetics
  • Flowers / growth & development*
  • Flowers / metabolism
  • Gibberellins / metabolism*
  • Immunoprecipitation
  • Photoperiod*
  • Repressor Proteins / metabolism*
  • Signal Transduction
  • Transcription Factors / metabolism*
  • Two-Hybrid System Techniques


  • Arabidopsis Proteins
  • CCAAT-Binding Factor
  • CONSTANS protein, Arabidopsis
  • DNA-Binding Proteins
  • GAI protein, Arabidopsis
  • Gibberellins
  • NF-YB2 protein, Arabidopsis
  • RGA protein, Arabidopsis
  • Repressor Proteins
  • Transcription Factors