Cytoplasmic tail domain of glycoprotein B is essential for HHV-6 infection

Nora F Mahmoud; Chyntia Jasirwan; Satoshi Kanemoto; Aika Wakata; Bochao Wang; Yuuki Hata; Satoshi Nagamata; Akiko Kawabata; Huamin Tang; Yasuko Mori

doi:10.1016/j.virol.2015.12.018

Cytoplasmic tail domain of glycoprotein B is essential for HHV-6 infection

Virology. 2016 Mar:490:1-5. doi: 10.1016/j.virol.2015.12.018. Epub 2016 Jan 21.

Authors

Affiliations

¹ Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan; Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
² Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan; Division of Hepatobiliary, Department of Internal Medicine, Faculty of Medicine, University of Indonesia, Indonesia.
³ Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan.
⁴ Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan; Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Japan.
⁵ Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan; Department of Immunology, Nanjing Medical University, Nanjing, China.
⁶ Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan. Electronic address: ymori@med.kobe-u.ac.jp.

PMID: 26802210
DOI: 10.1016/j.virol.2015.12.018

Abstract

Human herpesvirus 6 (HHV-6) glycoprotein B (gB) is an abundantly expressed viral glycoprotein required for viral entry and cell fusion, and is highly conserved among herpesviruses. The present study examined the function of HHV-6 gB cytoplasmic tail domain (CTD). A gB CTD deletion mutant was constructed which, in contrast to its revertant, could not be reconstituted. Moreover, deletion of gB cytoplasmic tail impaired the intracellular transport of gB protein to the trans-Golgi network (TGN). Taken together, these results suggest that gB CTD is critical for HHV-6 propagation and important for intracellular transportation.

Keywords: Cytoplasmic tail domain; Glycoprotein B; HHV-6; Intracellular transport.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Herpesvirus 6, Human / chemistry
Herpesvirus 6, Human / genetics
Herpesvirus 6, Human / growth & development
Herpesvirus 6, Human / metabolism*
Humans
Protein Structure, Tertiary
Roseolovirus Infections / virology*
Viral Envelope Proteins / chemistry*
Viral Envelope Proteins / genetics
Viral Envelope Proteins / metabolism*
trans-Golgi Network / virology

Substances

Viral Envelope Proteins
glycoprotein B, human herpesvirus-6