Reactive oxygen species regulatory mechanisms associated with rapid response of MC3T3-E1 cells for vibration stress

Biochem Biophys Res Commun. 2016 Feb 12;470(3):510-515. doi: 10.1016/j.bbrc.2016.01.120. Epub 2016 Jan 21.

Abstract

Although many previous studies have shown that refractory period-dependent memory effect of vibration stress is anabolic for skeletal homeostasis, little is known about the rapid response of osteoblasts simply derived from vibration itself. In view of the potential role of reactive oxygen species (ROS) in mediating differentiated activity of osteoblasts, whether and how ROS regulates the rapid effect of vibration deserve to be demonstrated. Our findings indicated that MC3T3-E1 cells underwent decreased gene expression of Runx2, Col-I and ALP and impaired ALP activity accompanied by increased mitochondrial fission immediately after vibration loading. Moreover, we also revealed the involvement of ERK-Drp1 signal transduction in ROS regulatory mechanisms responsible for the rapid effect of vibration stress.

Keywords: Drp1; Mitochondrial morphology; Osteogenic differentiation; ROS; Vibration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Adaptation, Physiological / physiology*
  • Animals
  • MAP Kinase Signaling System / physiology
  • Mechanotransduction, Cellular / physiology*
  • Mice
  • Mitochondria / physiology*
  • Osteogenesis / physiology*
  • Reactive Oxygen Species / metabolism*
  • Stress, Mechanical
  • Stress, Physiological / physiology*
  • Vibration

Substances

  • Reactive Oxygen Species