Click-Crosslinked Injectable Gelatin Hydrogels

Adv Healthc Mater. 2016 Mar 9;5(5):541-7. doi: 10.1002/adhm.201500757. Epub 2016 Jan 25.


Injectable gelatin hydrogels formed with bioorthogonal click chemistry (ClickGel) are cell-responsive ECM mimics for in vitro and in vivo biomaterials applications. Gelatin polymers with pendant norbornene (GelN) or tetrazine (GelT) groups can quickly and spontaneously crosslink upon mixing, allowing for high viability of encapsulated cells, establishment of 3D elongated cell morphologies, and biodegradation when injected in vivo.

Keywords: click chemistry; degradable; gelatin; hydrogels; injectable.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 3T3 Cells
  • Animals
  • Cell Adhesion
  • Cell Proliferation
  • Cell Shape
  • Click Chemistry / methods*
  • Cross-Linking Reagents / chemistry*
  • Female
  • Gelatin / chemistry*
  • Hydrogels / chemistry*
  • Mice
  • Subcutaneous Tissue


  • Cross-Linking Reagents
  • Hydrogels
  • Gelatin