Role of Periostin in Adhesion and Migration of Bone Remodeling Cells

PLoS One. 2016 Jan 25;11(1):e0147837. doi: 10.1371/journal.pone.0147837. eCollection 2016.

Abstract

Periostin is an extracellular matrix protein highly expressed in collagen-rich tissues subjected to continuous mechanical stress. Functionally, periostin is involved in tissue remodeling and its altered function is associated to numerous pathological processes. In orthodontics, periostin plays key roles in the maintenance of dental tissues and it is mainly expressed in those areas where tension or pressing forces are taking place. In this regard, high expression of periostin is essential to promote migration and proliferation of periodontal ligament fibroblasts. However little is known about the participation of periostin in migration and adhesion processes of bone remodeling cells. In this work we employ the mouse pre-osteoblastic MC3T3-E1 and the macrophage-like RAW 264.7 cell lines to overexpress periostin and perform different cell-based assays to study changes in cell behavior. Our data indicate that periostin overexpression not only increases adhesion capacity of MC3T3-E1 cells to different matrix proteins but also hampers their migratory capacity. Changes on RNA expression profile of MC3T3-E1 cells upon periostin overexpression have been also analyzed, highlighting the alteration of genes implicated in processes such as cell migration, adhesion or bone metabolism but not in bone differentiation. Overall, our work provides new evidence on the impact of periostin in osteoblasts physiology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Remodeling / genetics
  • Bone Remodeling / physiology
  • Cell Adhesion / genetics
  • Cell Adhesion / physiology
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / metabolism*
  • Cell Differentiation / genetics
  • Cell Differentiation / physiology
  • Cell Line
  • Cell Movement / genetics
  • Cell Movement / physiology
  • Mice

Substances

  • Cell Adhesion Molecules
  • Postn protein, mouse

Grant support

This work was supported by Fundación Universidad de Oviedo and Instituto Asturiano de Odontología. C.G.V. was financed by Fundación Universidad de Oviedo and L.S. was a recipient of Beca de Colaboración from Ministerio de Educación, Cultura y Deporte of Spain. This work was also partially supported by a grant from the Instituto de Salud Carlos III (Spain) (FISS PI11/00371) and Fondos FEDER. The Instituto Universitario de Oncología is supported by Obra Social Cajastur.