An engineered multicomponent bone marrow niche for the recapitulation of hematopoiesis at ectopic transplantation sites

J Hematol Oncol. 2016 Jan 25;9:4. doi: 10.1186/s13045-016-0234-9.

Abstract

Background: Bone marrow (BM) niches are often inaccessible for controlled experimentation due to their difficult accessibility, biological complexity, and three-dimensional (3D) geometry.

Methods: Here, we report the development and characterization of a BM model comprising of cellular and structural components with increased potential for hematopoietic recapitulation at ectopic transplantation sites. Cellular components included mesenchymal stromal cells (MSCs) and hematopoietic stem and progenitor cells (HSPCs). Structural components included 3D β-tricalcium phosphate (β-TCP) scaffolds complemented with Matrigel or collagen I/III gels for the recreation of the osteogenic/extracellular character of native BM.

Results: In vitro, β-TCP/Matrigel combinations robustly maintained proliferation, osteogenic differentiation, and matrix remodeling capacities of MSCs and maintenance of HSPCs function over time. In vivo, scaffolds promoted strong and robust recruitment of hematopoietic cells to sites of ectopic transplantation, vascularization, and soft tissue formation.

Conclusions: Our tissue-engineered BM system is a powerful tool to explore the regulatory mechanisms of hematopoietic stem and progenitor cells for a better understanding of hematopoiesis in health and disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow / metabolism
  • Bone Marrow / physiology*
  • Bone Marrow Cells / physiology*
  • Calcium Phosphates / metabolism
  • Cell Differentiation / physiology
  • Cell Proliferation / physiology
  • Cells, Cultured
  • Collagen / metabolism
  • Drug Combinations
  • Hematopoiesis / physiology*
  • Hematopoietic Stem Cells / physiology
  • Humans
  • Laminin
  • Mesenchymal Stem Cells / physiology
  • Mice, Inbred C57BL
  • Microscopy, Electron
  • Osteogenesis / physiology
  • Proteoglycans
  • Reproducibility of Results
  • Stem Cell Niche / physiology*
  • Stem Cell Transplantation / methods
  • Tissue Engineering / methods*
  • Tissue Scaffolds

Substances

  • Calcium Phosphates
  • Drug Combinations
  • Laminin
  • Proteoglycans
  • beta-tricalcium phosphate
  • matrigel
  • Collagen