Volta phase plate cryo-EM of the small protein complex Prx3

Nat Commun. 2016 Jan 28;7:10534. doi: 10.1038/ncomms10534.

Abstract

Cryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre resolution cryo-EM structures are rare compared with crystal structure depositions, particularly for relatively small particles (<400 kDa). Here we demonstrate the benefits of Volta phase plates for single-particle analysis by time-efficient cryo-EM structure determination of 257 kDa human peroxiredoxin-3 dodecamers at 4.4 Å resolution. The Volta phase plate improves the applicability of cryo-EM for small molecules and accelerates structure determination.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cryoelectron Microscopy / instrumentation
  • Cryoelectron Microscopy / methods*
  • Humans
  • Multiprotein Complexes / chemistry*
  • Peroxiredoxin III / chemistry*

Substances

  • Multiprotein Complexes
  • PRDX3 protein, human
  • Peroxiredoxin III