Production of Virus-Like Particles for Vaccination

Christine M Thompson; Marc G Aucoin; Amine A Kamen

doi:10.1007/978-1-4939-3043-2_14

Production of Virus-Like Particles for Vaccination

Methods Mol Biol. 2016:1350:299-315. doi: 10.1007/978-1-4939-3043-2_14.

Authors

Christine M Thompson^{1

2}, Marc G Aucoin³, Amine A Kamen^{2

4}

Affiliations

¹ Department of Chemical Engineering, Ecole Polytechnique de Montreal, 2500, Chemin de Polytechnique, Montreal, QC, Canada.
² National Research Council Canada, Montreal, QC, Canada.
³ Department of Chemical Engineering, University of Waterloo, 200 University Ave W, Waterloo, ON, Canada, N2L 3G1. marc.aucoin@uwaterloo.ca.
⁴ Department of Bioengineering, McGill University, Montreal, QC, Canada.

PMID: 26820864
DOI: 10.1007/978-1-4939-3043-2_14

Abstract

The ability to make a large variety of virus-like particles (VLPs) has been successfully achieved in the baculovirus expression vector system (BEVS)/insect cell system. The production and scale-up of these particles, which are mostly sought as vaccine candidates, are currently being addressed. Furthermore, these VLPs are being investigated as delivery agents for use as therapeutics. The use of host insect cells allows mass production of VLPs in a proven scalable system.

Keywords: Baculovirus; Insect cells; VLP; Viral vector; Virus-like particle.

MeSH terms

Animals
Baculoviridae / genetics
Bioreactors / virology
Culture Techniques
Electrophoresis, Polyacrylamide Gel
Genetic Engineering / methods*
Microscopy, Electron
Sf9 Cells
Spodoptera
Staining and Labeling
Vaccination*
Vaccines, Virus-Like Particle / biosynthesis*
Vaccines, Virus-Like Particle / genetics*
Vaccines, Virus-Like Particle / immunology

Substances

Vaccines, Virus-Like Particle