The neuronal response to injury as visualized by immunostaining of class III beta-tubulin in the rat

Neurosci Lett. 1989 Jul 31;102(2-3):137-41. doi: 10.1016/0304-3940(89)90068-2.


The neuronal response to trauma of the brain and spinal cord was examined by staining sections of injured central nervous system (CNS) with a monoclonal antibody (TuJ1) that recognizes class III beta-tubulin exclusively. Because class III beta-tubulin is expressed by neurons and not by glia, this monoclonal antibody stains neuronal cell bodies, dendrites, axons and axonal terminations darkly with a pale staining background. Thus, the TuJ1 antibody is extremely useful, revealing the fine details of axons and their terminations, as well as significant injury-related alterations in the composition of the somatic cytoskeleton.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Axons / analysis*
  • Axons / ultrastructure
  • Brain / ultrastructure*
  • Brain Chemistry
  • Immunoenzyme Techniques
  • Nerve Degeneration
  • Neurons / analysis*
  • Neurons / ultrastructure
  • Rats
  • Rats, Inbred Strains
  • Tubulin / analysis*


  • Antibodies, Monoclonal
  • Tubulin