TruSPAdes: barcode assembly of TruSeq synthetic long reads

Nat Methods. 2016 Mar;13(3):248-50. doi: 10.1038/nmeth.3737. Epub 2016 Feb 1.


The recently introduced TruSeq synthetic long read (TSLR) technology generates long and accurate virtual reads from an assembly of barcoded pools of short reads. The TSLR method provides an attractive alternative to existing sequencing platforms that generate long but inaccurate reads. We describe the truSPAdes algorithm ( for TSLR assembly and show that it results in a dramatic improvement in the quality of metagenomics assemblies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms*
  • Base Sequence
  • Chromosome Mapping / methods*
  • DNA Barcoding, Taxonomic / methods*
  • Gene Library
  • High-Throughput Nucleotide Sequencing / methods*
  • Molecular Sequence Data
  • Sequence Alignment / methods
  • Sequence Analysis, DNA / methods*
  • Software*