Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Apr;157(4):1613-21.
doi: 10.1210/en.2015-1945. Epub 2016 Feb 1.

Persistent cAMP Signaling by Internalized LH Receptors in Ovarian Follicles


Persistent cAMP Signaling by Internalized LH Receptors in Ovarian Follicles

Sandra Lyga et al. Endocrinology. .


A crucial event in female reproduction occurs at midcycle, when a LH peak induces the final maturation of ovarian follicles. LH signals via a G protein-coupled receptor selectively expressed in the outermost follicular cell layers. However, how LH signals are relayed inside these cells and finally to the oocyte is incompletely understood. Here, we monitored LH signaling in intact ovarian follicles of transgenic mice expressing a fluorescent cAMP sensor. We found that LH stimulation induces 2 phases of cAMP signaling in all cell layers surrounding the oocyte. Interfering with LH receptor internalization abolished the second, persistent cAMP phase and partially inhibited oocyte meiosis resumption. These data suggest that persistent cAMP signals from internalized LH receptors contribute to transmitting LH effects inside follicle cells and ultimately to the oocyte. Thus, this study indicates that the recently proposed paradigm of cAMP signaling by internalized G protein-coupled receptors is implicated in receptor function and is physiologically relevant.

Similar articles

See all similar articles

Cited by 17 articles

See all "Cited by" articles

Publication types

LinkOut - more resources