Proteome-wide Survey of the Autoimmune Target Repertoire in Autoimmune Polyendocrine Syndrome Type 1

Sci Rep. 2016 Feb 1;6:20104. doi: 10.1038/srep20104.

Abstract

Autoimmune polyendocrine syndrome type 1 (APS1) is a monogenic disorder that features multiple autoimmune disease manifestations. It is caused by mutations in the Autoimmune regulator (AIRE) gene, which promote thymic display of thousands of peripheral tissue antigens in a process critical for establishing central immune tolerance. We here used proteome arrays to perform a comprehensive study of autoimmune targets in APS1. Interrogation of established autoantigens revealed highly reliable detection of autoantibodies, and by exploring the full panel of more than 9000 proteins we further identified MAGEB2 and PDILT as novel major autoantigens in APS1. Our proteome-wide assessment revealed a marked enrichment for tissue-specific immune targets, mirroring AIRE's selectiveness for this category of genes. Our findings also suggest that only a very limited portion of the proteome becomes targeted by the immune system in APS1, which contrasts the broad defect of thymic presentation associated with AIRE-deficiency and raises novel questions what other factors are needed for break of tolerance.

Publication types

  • Clinical Trial
  • Multicenter Study
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Neoplasm / blood
  • Antigens, Neoplasm / immunology*
  • Autoantibodies / blood
  • Autoantibodies / immunology*
  • Autoantigens / blood
  • Autoantigens / immunology*
  • Female
  • Humans
  • Male
  • Neoplasm Proteins / blood
  • Neoplasm Proteins / immunology*
  • Polyendocrinopathies, Autoimmune / blood
  • Polyendocrinopathies, Autoimmune / immunology*
  • Protein Array Analysis
  • Protein Disulfide-Isomerases / blood
  • Protein Disulfide-Isomerases / immunology*
  • Proteome / immunology*
  • Proteome / metabolism
  • Scandinavian and Nordic Countries

Substances

  • Antigens, Neoplasm
  • Autoantibodies
  • Autoantigens
  • MAGEB2 protein, human
  • Neoplasm Proteins
  • Proteome
  • PDILT protein, human
  • Protein Disulfide-Isomerases

Supplementary concepts

  • Autoimmune polyendocrinopathy syndrome, type 1