In vivo volumetric imaging of biological dynamics in deep tissue via wavefront engineering

Opt Express. 2016 Jan 25;24(2):1214-21. doi: 10.1364/OE.24.001214.


Biological systems undergo dynamical changes continuously which span multiple spatial and temporal scales. To study these complex biological dynamics in vivo, high-speed volumetric imaging that can work at large imaging depth is highly desired. However, deep tissue imaging suffers from wavefront distortion, resulting in reduced Strehl ratio and image quality. Here we combine the two wavefront engineering methods developed in our lab, namely the optical phase-locked ultrasound lens based volumetric imaging and the iterative multiphoton adaptive compensation technique, and demonstrate in vivo volumetric imaging of microglial and mitochondrial dynamics at large depth in mouse brain cortex and lymph node, respectively.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cerebral Cortex / cytology*
  • Imaging, Three-Dimensional / methods*
  • Lymph Nodes / cytology
  • Lymphocytes / cytology
  • Mice
  • Microglia / cytology
  • Mitochondria / metabolism
  • Optics and Photonics / methods*