Alumina-phosphate complexes for immobilization of biomolecules

Anal Biochem. 1989 Jul;180(1):1-10. doi: 10.1016/0003-2697(89)90080-8.

Abstract

Organic compounds containing the -PO3H2 function are strongly and specifically adsorbed by aluminum oxide in water within a large range of pH. The reversible character of the interaction allows the adsorbed organic phosphates to be displaced by inorganic phosphate buffers resulting in their purification by an affinity-like chromatographic procedure. The interaction between alumina and selected multifunctional compounds containing a phosphonate group yields a chemically activated alumina-phosphate complex onto which enzymes or other molecules can be immobilized. A number of proteases immobilized on alumina through such phosphate interactions proved to be active in the presence of organic solvents. As a consequence, enzyme-catalyzed peptide synthesis in a water-limited environment and optical resolution of amino acids in water-organic solvent emulsions can be accomplished.

Publication types

  • Review

MeSH terms

  • Adsorption
  • Aluminum Oxide*
  • Aluminum*
  • Amino Acids / isolation & purification
  • Animals
  • Chemical Phenomena
  • Chemistry
  • Chromatography, Affinity
  • Enzymes, Immobilized*
  • Peptide Biosynthesis
  • Phosphoric Acids*
  • Stereoisomerism

Substances

  • Amino Acids
  • Enzymes, Immobilized
  • Phosphoric Acids
  • Aluminum
  • Aluminum Oxide