Graphene oxide for rapid determination of testosterone in the presence of cetyltrimethylammonium bromide in urine and blood plasma of athletes

Mater Sci Eng C Mater Biol Appl. 2016 Apr 1;61:246-50. doi: 10.1016/j.msec.2015.12.005. Epub 2015 Dec 4.


Electro-reduction behavior of testosterone at reduced graphene oxide/glassy carbon electrode (rGO/GCE) was studied. Cationic surfactant cetyltrimethylammonium bromide (CTAB) enhanced the reduction peak of testosterone. In borate buffer (pH5.4) CTAB-testosterone showed a reduction peak at -1.1 V (versus, Ag/AgCl). The increment of peak current obtained by deducting the reduction peak current of the CTAB-testosterone was rectilinear with testosterone concentration in the range of 2.0 to 210.0 nM, with a detection limit of 0.1 nM. The sensor was used for quantification of testosterone in biological fluids and drug.

Keywords: Biological fluids; Cetyltrimethylammonium bromide; Graphene; Testosterone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Athletes*
  • Cetrimonium
  • Cetrimonium Compounds / chemistry*
  • Female
  • Graphite / chemistry*
  • Humans
  • Male
  • Sensitivity and Specificity
  • Testosterone / blood*
  • Testosterone / urine*


  • Cetrimonium Compounds
  • Testosterone
  • Graphite
  • Cetrimonium