Ingestion of bioactive collagen hydrolysates enhance facial skin moisture and elasticity and reduce facial ageing signs in a randomised double-blind placebo-controlled clinical study

J Sci Food Agric. 2016 Sep;96(12):4077-81. doi: 10.1002/jsfa.7606. Epub 2016 Feb 10.


Background: Several human studies have demonstrated occurrence of two major collagen peptides, prolyl-hydroxyproline (Pro-Hyp) and hydroxyprolyl-glycine (Hyp-Gly), in human peripheral blood. Some in vitro studies have demonstrated that Pro-Hyp and Hyp-Gly exert chemotaxis on dermal fibroblasts and enhance cell proliferation. Additionally, Pro-Hyp enhances the production of hyaluronic acid by dermal fibroblasts. These findings suggest that the amounts of Pro-Hyp and Hyp-Gly in blood are important factors to show the efficacy of collagen hydrolysates on skin health.

Results: We conducted a randomised double-blind placebo-controlled clinical trial of ingestion of two types of collagen hydrolysates, which are composed of different amounts of the bioactive dipeptides Pro-Hyp and Hyp-Gly, to investigate their effects on the improvement of skin conditions. Improvement in skin conditions, such as skin moisture, elasticity, wrinkles, and roughness, were compared with a placebo group at baseline, and 4 and 8 weeks after the start of the trial. In addition, the safety of dietary supplementation with these peptides was evaluated by blood test. Collagen hydrolysate with a higher content of bioactive collagen peptides (H-CP) showed significant and more improvement than the collagen hydrolysate with a lower content of bioactive collagen peptides (L-CP) and the placebo, in facial skin moisture, elasticity (R2), wrinkles and roughness, compared with the placebo group. In addition, there were no adverse events during the trial.

Conclusion: This study demonstrated that the use of the collagen hydrolysate with a higher content of Pro-Hyp and Hyp-Gly led to more improvement in facial skin conditions, including facial skin moisture, elasticity, wrinkles and roughness. © 2016 Society of Chemical Industry.

Keywords: bioactive peptides; clinical study; collagen hydrolysate; collagen peptide; skin health; skin roughness.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Administration, Oral
  • Adult
  • Collagen Type I / administration & dosage
  • Collagen Type I / pharmacology*
  • Dietary Supplements
  • Dipeptides / administration & dosage
  • Dipeptides / pharmacology
  • Double-Blind Method
  • Female
  • Humans
  • Middle Aged
  • Oligopeptides / administration & dosage
  • Oligopeptides / pharmacology
  • Skin Aging*
  • Skin Physiological Phenomena / drug effects*
  • Treatment Outcome


  • Collagen Type I
  • Dipeptides
  • Oligopeptides
  • glycyl-prolyl-hydroxyproline
  • hydroxyprolyl-glycine