Estrogen and progesterone receptor assays, by the EIA method (Abbott), of low-speed supernates of breast-cancer homogenates

Clin Chem. 1989 Nov;35(11):2226-7.

Abstract

Estrogen receptor (ER) and progesterone receptor (PR) assays were performed by solid-phase enzyme immunoassays (Abbott) on low-speed (2600 x g) and high-speed (100,000 x g) supernates of breast-cancer homogenates. The results were similar, indicating that ultracentrifugation is not essential for ER and PR assays with the Abbott kit.

Publication types

  • Comparative Study

MeSH terms

  • Breast Neoplasms / analysis*
  • Centrifugation
  • Cytosol / analysis
  • Humans
  • Immunoenzyme Techniques*
  • Quality Control
  • Reagent Kits, Diagnostic
  • Receptors, Estrogen / analysis*
  • Receptors, Progesterone / analysis*

Substances

  • Reagent Kits, Diagnostic
  • Receptors, Estrogen
  • Receptors, Progesterone