Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells

Angew Chem Int Ed Engl. 2016 Mar 1;55(10):3290-4. doi: 10.1002/anie.201511018. Epub 2016 Feb 4.

Abstract

A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500-630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60 nm.

Keywords: carbopyronines; fluorescence; living cells; optical microscopy; rhodamines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescent Dyes / chemistry
  • HeLa Cells
  • Humans
  • Microscopy / methods*
  • Pyronine / chemistry*
  • Rhodamines / chemistry*

Substances

  • Fluorescent Dyes
  • Rhodamines
  • Pyronine