Sequestering survivin to functionalized nanoparticles: a strategy to enhance apoptosis in cancer cells

Biomater Sci. 2016 Apr;4(4):614-26. doi: 10.1039/c5bm00580a. Epub 2016 Feb 4.

Abstract

Survivin belongs to the family of inhibitor of apoptosis proteins (IAP) and is present in most cancers while being below detection limits in most terminally differentiated adult tissues, making it an attractive protein to target for diagnostic and, potentially, therapeutic roles. Sub-100 nm poly(propargyl acrylate) (PA) particles were surface modified through the copper-catalyzed azide/alkyne cycloaddition of an azide-terminated survivin ligand derivative (azTM) originally proposed by Abbott Laboratories and speculated to bind directly to survivin (protein) at its dimer interface. Using affinity pull-down studies, it was determined that the PA/azTM nanoparticles selectively bind survivin and the particles can enhance apoptotic cell death in glioblastoma cell lines and other survivin over-expressing cell lines such as A549 and MCF7 relative to cells incubated with the original Abbott-derived small molecule inhibitor.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Acrylates / chemistry*
  • Apoptosis* / physiology
  • Azides / chemistry*
  • Azides / pharmacology
  • Catalysis
  • Cell Line, Tumor
  • Copper / chemistry
  • Cycloaddition Reaction
  • Humans
  • Inhibitor of Apoptosis Proteins / chemistry*
  • Inhibitor of Apoptosis Proteins / metabolism
  • Inhibitor of Apoptosis Proteins / pharmacology
  • Ligands
  • Microtubule-Associated Proteins / chemistry
  • Microtubule-Associated Proteins / metabolism
  • Nanoparticles / chemistry*
  • Neoplasm Proteins / chemistry*
  • Neoplasm Proteins / metabolism
  • Polymers / chemistry*

Substances

  • Acrylates
  • Azides
  • Inhibitor of Apoptosis Proteins
  • Ligands
  • Microtubule-Associated Proteins
  • Neoplasm Proteins
  • Polymers
  • poly(propyl acrylic acid)
  • Copper