Expression of aryl hydrocarbon receptor and ATG16L1 protein in experimental oxazolone-induced colitis in rats

Fiziol Zh. 2015;61(5):57-64. doi: 10.15407/fz61.05.057.

Abstract

We studied the expression of AhR and ATG16L1 protein in experimental oxazolone-induced colitis in rats and anti-inflammatory action of recombinant antagonist of IL-1 receptors (ARIL-1) and simvastatin. The immunopositive cells were determined using an indirect immunofluorescence technique with using a monoclonal rat antibody. It has been established that development of colitis was accompanied by an increase of total number of ATG16L1-lymphocytes (by 30%, P < 0.05) in lymphoid structures of the colon. However the amount of AhR(+)-lymphocytes has not changed. At the same time has increased the concentration of ATG16L1 protein (by 4-11%, P < 0.05) in immunopositive cells. Administration of simvastatin and ARIL-1 during the development of experimental pathology was accompanied by decrease of total number of AhR(+) (by 24-38%, P < 0.05) and ATG16L1(+)-lymphocytes (by 43% - 2 fold, P < 0.05) in the colon.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Antibodies, Monoclonal / chemistry
  • Autophagy-Related Proteins
  • Carrier Proteins / genetics*
  • Carrier Proteins / immunology
  • Colitis / chemically induced
  • Colitis / drug therapy*
  • Colitis / immunology
  • Colitis / pathology
  • Colon / drug effects
  • Colon / immunology
  • Colon / metabolism*
  • Colon / pathology
  • Gene Expression Regulation
  • Immunophenotyping
  • Lymphocyte Count
  • Lymphocytes / drug effects
  • Lymphocytes / immunology
  • Lymphocytes / pathology
  • Male
  • Oxazolone
  • Peptides / metabolism
  • Peptides / pharmacology
  • Rats
  • Rats, Wistar
  • Receptors, Aryl Hydrocarbon / genetics*
  • Receptors, Aryl Hydrocarbon / immunology
  • Receptors, Interleukin-1 / antagonists & inhibitors
  • Receptors, Interleukin-1 / genetics
  • Receptors, Interleukin-1 / immunology
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / pharmacology
  • Simvastatin / pharmacology

Substances

  • ATG16L1 protein, human
  • Anti-Inflammatory Agents
  • Antibodies, Monoclonal
  • Autophagy-Related Proteins
  • Carrier Proteins
  • Peptides
  • Receptors, Aryl Hydrocarbon
  • Receptors, Interleukin-1
  • Recombinant Proteins
  • Oxazolone
  • Simvastatin