Amplification, mutation and selection of catalytic RNA

G F Joyce

doi:10.1016/0378-1119(89)90033-4

Amplification, mutation and selection of catalytic RNA

Gene. 1989 Oct 15;82(1):83-7. doi: 10.1016/0378-1119(89)90033-4.

Author

G F Joyce¹

Affiliation

¹ Salk Institute for Biological Studies, La Jolla, CA 92037.

PMID: 2684778
DOI: 10.1016/0378-1119(89)90033-4

Abstract

RNA, by virtue of its genotypic and phenotypic properties, is a suitable substrate for molecular evolution in the laboratory. We have developed techniques for the rapid amplification, mutation and selection of catalytic RNA. By combining these techniques in an iterative fashion, we are attempting to construct an RNA-based evolving system. Such a system could be used to explore the catalytic potential of RNA.

MeSH terms

Biological Evolution
Catalysis
Gene Amplification
Genetic Techniques*
Mutation
Nucleic Acid Conformation
RNA, Catalytic
RNA, Ribosomal / genetics*
Selection, Genetic

Substances

RNA, Catalytic
RNA, Ribosomal