The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth

Gene. 1989 Nov 30;83(2):271-9. doi: 10.1016/0378-1119(89)90113-3.


In Saccharomyces cerevisiae, the SCL-1 mutation is a dominant suppressor of the cycloheximide-resistant, temperature-sensitive (ts) lethal mutation, crl3 [McCusker and Haber, Genetics 119 (1988a) 303-315]. The wild-type scl1+ gene was isolated by screening subclones of the 35-kb region between TRP5 and LEU1 for restoration of the ts phenotype in an SCL1-1 crl3-2 strain. The scl1+ mRNA is about 900 nt long and encodes an open reading frame of 810 bp. The polypeptide deduced from scl1+ possesses a putative secretory signal peptide. The 5'-noncoding region may be under multiple controls, since it contains significant homology to the consensus sequences for the DNA-binding proteins, GCN4, GFI and, possibly, TUF. Gene disruption of scl1+ demonstrates that it is an essential gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification
  • Genes, Dominant
  • Genes, Fungal*
  • Genes, Lethal
  • Molecular Sequence Data
  • Mutation
  • Plasmids
  • RNA, Fungal / genetics
  • RNA, Fungal / isolation & purification
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Suppression, Genetic*
  • Transformation, Genetic


  • DNA, Fungal
  • RNA, Fungal