Gold-Quantum Dot Core-Satellite Assemblies for Lighting Up MicroRNA In Vitro and In Vivo

Xueli Zhao; Liguang Xu; Maozhong Sun; Wei Ma; Xiaoling Wu; Hua Kuang; Libing Wang; Chuanlai Xu

doi:10.1002/smll.201503629

Gold-Quantum Dot Core-Satellite Assemblies for Lighting Up MicroRNA In Vitro and In Vivo

Small. 2016 Sep;12(34):4662-8. doi: 10.1002/smll.201503629. Epub 2016 Feb 5.

Authors

Xueli Zhao¹, Liguang Xu¹, Maozhong Sun¹, Wei Ma¹, Xiaoling Wu¹, Hua Kuang², Libing Wang¹, Chuanlai Xu¹

Affiliations

¹ State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
² State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China. kuangh@jiangnan.edu.cn.

PMID: 26849492
DOI: 10.1002/smll.201503629

Abstract

A high yield DNA-driven gold-quantum dot core-satellite is developed for miRNA detection in vitro and vivo. In the presence of the target miRNA, the DNA hairpin between core and satellite is ruined, resulting in the recovery of fluorescence. The limit of detection for miRNA-21 detection in living cells reaches 296 copies per cell.

Keywords: core-satellite assemblies; detection; fluorescence; microRNA; self-assembly.

MeSH terms

Animals
Cell Line, Tumor
Gold / chemistry*
Humans
Mice
MicroRNAs / metabolism*
Quantum Dots / chemistry*
Quantum Dots / ultrastructure

Substances

MIRN21 microRNA, human
MicroRNAs
Gold