α-Bisabolol Inhibits Invasiveness and Motility in Pancreatic Cancer Through KISS1R Activation

Anticancer Res. 2016 Feb;36(2):583-9.

Abstract

α-Bisabolol is a plant-derived, oily sesquiterpene alcohol that induces apoptosis of various cancer cells. We previously reported the antiproliferative effects of α-bisabolol on pancreatic cancer cell lines using in vitro and in vivo experiments. However, the effects of α-bisabolol on tumor invasiveness and motility are still unknown. In this study, demonstrated that α-bisabolol suppressed the invasiveness and motility of a pancreatic cancer cell line. Although Early growth response 1 (EGR1) was involved in antiproliferative effects of α-bisabolol, it had no relationship with the inhibitory effect of α-bisabolol on cellular invasiveness and motility. Polymerase chain reaction analysis revealed that α-bisabolol induced Kisspeptin 1 receptor (KISS1R) in pancreatic cancer cell lines. The inhibition of KISS1R weakened the inhibitory effect of α-bisabolol on invasiveness of pancreatic cancer cells. The results also implied that the inhibitory effects of α-bisabolol on tumor invasiveness and motility are at least partly associated with the activation of KISS1R. However, there is a possibility that other molecular mechanisms of α-bisabolol regulate invasiveness and motility in pancreatic cancer cells. Further investigations are necessary to clarify the precise mechanisms of α-bisabolol activity for clinical application as a novel treatment for pancreatic cancer.

Keywords: KISS1R; KISS1R siRNA; metastasis-suppressor gene; pancreatic cancer; α-Bisabolol.

MeSH terms

  • Apoptosis
  • Blotting, Western
  • Cell Movement / drug effects*
  • Cell Proliferation
  • Early Growth Response Protein 1 / antagonists & inhibitors
  • Early Growth Response Protein 1 / genetics
  • Early Growth Response Protein 1 / metabolism*
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Humans
  • Monocyclic Sesquiterpenes
  • Neoplasm Invasiveness
  • Pancreatic Neoplasms / drug therapy*
  • Pancreatic Neoplasms / metabolism
  • Pancreatic Neoplasms / pathology*
  • RNA, Messenger / genetics
  • RNA, Small Interfering / genetics
  • Real-Time Polymerase Chain Reaction
  • Receptors, G-Protein-Coupled / antagonists & inhibitors
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, G-Protein-Coupled / metabolism*
  • Receptors, Kisspeptin-1
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sesquiterpenes / pharmacology*
  • Tumor Cells, Cultured

Substances

  • EGR1 protein, human
  • Early Growth Response Protein 1
  • KISS1R protein, human
  • Monocyclic Sesquiterpenes
  • RNA, Messenger
  • RNA, Small Interfering
  • Receptors, G-Protein-Coupled
  • Receptors, Kisspeptin-1
  • Sesquiterpenes
  • bisabolol