Nano-biosensors to detect beta-amyloid for Alzheimer's disease management

Biosens Bioelectron. 2016 Jun 15;80:273-287. doi: 10.1016/j.bios.2016.01.065. Epub 2016 Jan 28.

Abstract

Beta-amyloid (β-A) peptides are potential biomarkers to monitor Alzheimer's diseases (AD) for diagnostic purposes. Increased β-A level is neurotoxic and induces oxidative stress in brain resulting in neurodegeneration and causes dementia. As of now, no sensitive and inexpensive method is available for β-A detection under physiological and pathological conditions. Although, available methods such as neuroimaging, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) detect β-A, but they are not yet extended at point-of-care (POC) due to sophisticated equipments, need of high expertize, complicated operations, and challenge of low detection limit. Recently, β-A antibody based electrochemical immuno-sensing approach has been explored to detect β-A at pM levels within 30-40 min compared to 6-8h of ELISA test. The introduction of nano-enabling electrochemical sensing technology could enable rapid detection of β-A at POC and may facilitate fast personalized health care delivery. This review explores recent advancements in nano-enabling electrochemical β-A sensing technologies towards POC application to AD management. These analytical tools can serve as an analytical tool for AD management program to obtain bio-informatics needed to optimize therapeutics for neurodegenerative diseases diagnosis management.

Keywords: Alzheimer's diseases; Beta-amyloid; Diseases management; Electrochemical sensor; Point-of-care sensing.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Alzheimer Disease / diagnosis*
  • Amyloid beta-Peptides / chemistry
  • Amyloid beta-Peptides / isolation & purification*
  • Antibodies / chemistry
  • Biosensing Techniques / methods*
  • Brain / metabolism
  • Brain / pathology
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Limit of Detection
  • Peptide Fragments / chemistry
  • Peptide Fragments / isolation & purification*

Substances

  • Amyloid beta-Peptides
  • Antibodies
  • Peptide Fragments