Family-wide Structural Analysis of Human Numb-Associated Protein Kinases

Structure. 2016 Mar 1;24(3):401-11. doi: 10.1016/j.str.2015.12.015. Epub 2016 Feb 4.

Abstract

The highly diverse Numb-associated kinase (NAK) family has been linked to broad cellular functions including receptor-mediated endocytosis, Notch pathway modulation, osteoblast differentiation, and dendrite morphogenesis. Consequently, NAK kinases play a key role in a diverse range of diseases from Parkinson's and prostate cancer to HIV. Due to the plasticity of this kinase family, NAK kinases are often inhibited by approved or investigational drugs and have been associated with side effects, but they are also potential drug targets. The presence of cysteine residues in some NAK family members provides the possibility for selective targeting via covalent inhibition. Here we report the first high-resolution structures of kinases AAK1 and BIKE in complex with two drug candidates. The presented data allow a comprehensive structural characterization of the NAK kinase family and provide the basis for rational design of selective NAK inhibitors.

Keywords: AAK1; BIKE; Numb-associated kinase (NAK); activation segment; inhibitor selectivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • Conserved Sequence
  • Cysteine / metabolism
  • Humans
  • Intracellular Signaling Peptides and Proteins / metabolism
  • Models, Molecular
  • Protein Binding
  • Protein Kinase Inhibitors / metabolism
  • Protein-Serine-Threonine Kinases / chemistry*
  • Protein-Serine-Threonine Kinases / metabolism*
  • Transcription Factors / metabolism

Substances

  • Intracellular Signaling Peptides and Proteins
  • Protein Kinase Inhibitors
  • Transcription Factors
  • AAK1 protein, human
  • BMP2K protein, human
  • GAK protein, human
  • Protein-Serine-Threonine Kinases
  • STK16 protein, human
  • Cysteine