High frame-rate resolution of cell division during Candida albicans filamentation

Fungal Genet Biol. 2016 Mar;88:54-8. doi: 10.1016/j.fgb.2016.02.001. Epub 2016 Feb 4.

Abstract

The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1 frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR., while the migration pattern of sister nuclei differed between unbranched and branched hyphae. The presented movie challenges and extends previous concepts of C. albicans cell division.

Keywords: Hypha; Nuclear division; Septum.

Publication types

  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Aneuploidy
  • Candida albicans / genetics*
  • Candida albicans / growth & development*
  • Cell Division
  • Cell Nucleolus / genetics
  • Cell Nucleus / genetics
  • Cell Nucleus Division / genetics*
  • Humans
  • Hyphae / genetics
  • Hyphae / growth & development
  • Ploidies
  • Time-Lapse Imaging / instrumentation
  • Time-Lapse Imaging / methods
  • Virulence